Document Type : Original Article
Authors
1 Department of Basic Sciences, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran
2 Department of Pathology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran
3 Department of Internal Medicine, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran
Abstract
The purpose of the current study was to examine the effect of hydroalcoholic extract of Psidium guajava plant on bone marrow cells in rats and it will be extended to humans. Guava plant leaves were collected from Chabahar region in Sistan and Baluchistan province and dried. 40 adult male rats were assigned to one control group and three experimental groups (subjects that were administeredhydroalcoholic extract of P. guajava leaves with respective doses of 3000 mg/kg, 4000 mg/kg, or 5000 mg/kg, for 3 months, once daily). Following the intervention period, blood was taken from the heart and bone marrow was taken from the femur. Several parameters such as cell blood count, hemoglobin, and hematocrit, were examined in the blood sample. Also, in the bone marrow sample, a relative count was performed on five hundred bone marrow cells and the ratio of myeloid to erythroid was determined in both control and experimental groups. The results of this study indicated that the hydroalcoholic extract somewhat increased white blood cells and red blood cells, but no significant change was observed. The result of the study revealed the positive effects of guava leaf extract may be due to flavonoids, quercetin and triterpenes, which strong antioxidants that can prevent damage are caused by free radicals destroying cells. This study shows that guava leaf extract can have a positive effect on hematological parameters, as well as the lack of mutagenicity and cytotoxicity in high doses of this plant extract rather reflects its safe use in traditional medicine. Therefore, it is recommended that guava leaf extract be considered as a complementary and alternative treatment for many diseases, including the treatment of anemia.
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Zabol University of Medical Sciences
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- Kumar M, Tomar M, Amarowicz R, Saurabh V, Nair MS, Maheshwari C, Sasi M, Prajapati U, Hasan M, Singh S (2021) Guava (Psidium guajava L.) leaves: Nutritional composition, phytochemical profile, and health-promoting bioactivities. Foods 10 (4): 752. doi: https://doi.org/10.3390/foods10040752
- Angulo-López JE, Flores-Gallegos AC, Torres-León C, Ramírez-Guzmán KN, Martínez GA, Aguilar CN (2021) Guava (Psidium guajava L.) fruit and valorization of industrialization by-products. Processes 9 (6): 1075. doi: https://doi.org/10.3390/pr9061075
- Comazzetto S, Shen B, Morrison SJ (2021) Niches that regulate stem cells and hematopoiesis in adult bone marrow. Developmental cell 56 (13): 1848-1860. doi: https://doi.org/10.1016/j.devcel.2021.05.018
- Sarangthem V, Sharma H, Mendiratta M, Sahoo RK, Park R-W, Kumar L, Singh TD, Mohanty S (2022) Application of Bio-Active Elastin-like Polypeptide on Regulation of Human Mesenchymal Stem Cell Behavior. Biomedicines 10 (5): 1151. doi: https://doi.org/10.3390/biomedicines10051151
- Kelly BT, Pennington KM, Limper AH (2020) Advances in the diagnosis of fungal pneumonias. Expert review of respiratory medicine 14 (7): 703-714. doi: https://doi.org/10.1080/17476348.2020.1753506
- Michalowitz A, Yang J, Castaneda P, Litrenta J (2020) Existing and emerging methods of diagnosis and monitoring of pediatric musculoskeletal infection. Injury 51 (10): 2110-2117. doi: https://doi.org/10.1016/j.injury.2020.06.020
- Jun Ling S, Guang Yu Z, Li Hui S, Xiao Song L, Jun F, Zhen Fen Z (2023) Determination of bone marrow cell morphology in rat. Cellular and molecular biology (Noisy-le-Grand, France) 69 (11): 41-44. doi: https://doi.org/10.14715/cmb/2023.69.11.7
- Tuan HNA, Hai NDX, Thinh NT (2022) Shape Prediction of Nasal Bones by Digital 2D-Photogrammetry of the Nose Based on Convolution and Back-Propagation Neural Network. Computational and mathematical methods in medicine 2022: 5938493. doi: https://doi.org/10.1155/2022/5938493
- Afsar B, Kanbay M, Afsar RE (2022) Interconnections of fibroblast growth factor 23 and klotho with erythropoietin and hypoxia-inducible factor. Mol Cell Biochem 477 (7): 1973-1985. doi: https://doi.org/10.1007/s11010-022-04422-3
- Aprile A, Raggi L, Bolamperti S, Villa I, Storto M, Morello G, Marktel S, Tripodo C, Cappellini MD, Motta I, Rubinacci A, Ferrari G (2023) Inhibition of FGF23 is a therapeutic strategy to target hematopoietic stem cell niche defects in beta-thalassemia. Sci Transl Med 15 (698): eabq3679. doi: https://doi.org/10.1126/scitranslmed.abq3679
- Weiler S, Nairz M (2021) TAM-ing the CIA-Tumor-Associated Macrophages and Their Potential Role in Unintended Side Effects of Therapeutics for Cancer-Induced Anemia. Front Oncol 11: 627223. doi: https://doi.org/10.3389/fonc.2021.627223
- Akbari A, Nasiri K, Heydari M, Mosavat SH, Iraji A (2017) The protective effect of hydroalcoholic extract of Zingiber officinale Roscoe (Ginger) on ethanol-induced reproductive toxicity in male rats. Journal of evidence-based complementary & alternative medicine 22 (4): 609-617. doi: https://doi.org/10.1177/2156587216687696
- Jahanbani A, Goodarzi M, Sajjadi Dezfouli SM, Yourdkhani MR, Eskandari Roozbahani N (2023) A Novel Anticoagulation Method Based on Electrochemical Characteristics of Blood: An in vitro Study. Blood purification 52 (2): 122-131. doi: https://doi.org/10.1159/000526191
- Thériault M, Caillet S, Kermasha S, Lacroix M (2006) Antioxidant, antiradical and antimutagenic activities of phenolic compounds present in maple products. Food chemistry 98 (3): 490-501. doi: https://doi.org/10.1016/j.foodchem.2005.05.079
- Fazeli-Nasab B, Valizadeh M, Beigomi M (2022) Evaluation of Antioxidant and Antimicrobial Activity of Some Medicinal Plant Extracts on Escherichia coli Isolated from Poultry Feces. Journal of Medicinal plants and By-product 11 (2): 265-275. doi: https://doi.org/10.22092/jmpb.2021.353243.1319
- Fazeli-Nasab B, Ghafari M, Jahantigh M, Beigomi Z, Saeidi S (2023) Evaluation of Phenolic and Flavonoid Content, Alkaloids, Antioxidant Capacity and Antibacterial Properties of Methanolic Extract of Zahak Native Medicinal Plants Against Seven Pathogens. Journal of Medicinal plants and By-product 13 (1): 57-65. doi: https://doi.org/10.22034/jmpb.2023.128540
- Shahdadi F, Khorasani S, Salehi-Sardoei A, Fallahnajmabadi F, Fazeli-Nasab B, Sayyed RZ (2023) GC-MS profiling of Pistachio vera L., and effect of antioxidant and antimicrobial compounds of it's essential oil compared to chemical counterparts. Scientific Reports 13 (1): 21694. doi: https://doi.org/10.1038/s41598-023-48844-5
- Kaileh M, Berghe WV, Boone E, Essawi T, Haegeman G (2007) Screening of indigenous Palestinian medicinal plants for potential anti-inflammatory and cytotoxic activity. Journal of ethnopharmacology 113 (3): 510-516. doi: https://doi.org/10.1016/j.jep.2007.07.008
- Manosroi J, Dhumtanom P, Manosroi A (2006) Anti-proliferative activity of essential oil extracted from Thai medicinal plants on KB and P388 cell lines. Cancer letters 235 (1): 114-120. doi: https://doi.org/10.1016/j.canlet.2005.04.021
- Limsong J, Benjavongkulchai E, Kuvatanasuchati J (2004) Inhibitory effect of some herbal extracts on adherence of Streptococcus mutans. Journal of Ethnopharmacology 92 (2-3): 281-289. doi: https://doi.org/10.1016/j.jep.2004.03.008
- Chen K-C, Hsieh C-L, Peng C-C, Hsieh-Li H-M, Chiang H-S, Huang K-D, Peng RY (2007) Brain derived metastatic prostate cancer DU-145 cells are effectively inhibited in vitro by guava (Psidium gujava L.) leaf extracts. Nutrition and cancer 58 (1): 93-106. doi: https://doi.org/10.1080/01635580701308240
- Kovac J, Slobodnikova L, Trajcikova E, Rendekova K, Mucaji P, Sychrova A, Bittner Fialova S (2022) Therapeutic Potential of Flavonoids and Tannins in Management of Oral Infectious Diseases-A Review. Molecules 28 (1). doi: https://doi.org/10.3390/molecules28010158
- Petrisor G, Motelica L, Craciun LN, Oprea OC, Ficai D, Ficai A (2022) Melissa officinalis: Composition, Pharmacological Effects and Derived Release Systems-A Review. Int J Mol Sci 23 (7). doi: https://doi.org/10.3390/ijms23073591
- Saparbekova AA, Kantureyeva GO, Kudasova DE, Konarbayeva ZK, Latif AS (2023) Potential of phenolic compounds from pomegranate (Punica granatum L.) by-product with significant antioxidant and therapeutic effects: A narrative review. Saudi J Biol Sci 30 (2): 103553. doi: https://doi.org/10.1016/j.sjbs.2022.103553
- Uboh FE, Okon IE, Ekong MB (2010) Effect of aqueous extract of Psidium guajava leaves on liver enzymes, histological integrity and hematological indices in rats. Gastroenterology Research 3 (1): 32-38. doi: https://doi.org/10.4021%2Fgr2010.02.174w
- Sazmand M, Mehrabani D, Hosseini SE, Amini M (2018) The effect of hydroalcoholic extract of Cannabis Sativa on morphology and growth of bone marrow mesenchymal stem cells in rat. Electronic Journal of General Medicine 15 (3): em32. doi: https://doi.org/10.29333/ejgm/86195
- Raza AA, Mushtaq R, Khwaja S, Akram A, Karim A, Akhter A (2023) Antioxidant associated chemoprophylaxis effect of natural spice and green vegetable on hepatotoxicity. Brazilian journal of biology = Revista brasleira de biologia 84: e266940. doi: https://doi.org/10.1590/1519-6984.266940
- Silitonga M, Sinaga E, Nugrahalia M, Silitonga PM (2023) Hepatoprotective activity of ethanolic extract of Plectranthus amboinicus (lour.) spreng leaf in DMBA induced rats. Toxicon 232: 107212. doi: https://doi.org/10.1016/j.toxicon.2023.107212
- Tusubira D, Aja PM, Munezero J, Ssedyabane F, Namale N, Ifie JE, Agu PC, Ajayi CO, Okoboi J (2023) Safety profile of colocasia esculenta tuber extracts in benign prostate hyperplasia. BMC complementary medicine and therapies 23 (1): 187. doi: https://doi.org/10.1186/s12906-023-04018-4
- Barakat H, Alkhurayji RI, Aljutaily T (2023) Immune-Boosting Potentiating Properties of Brassica nigra Hydroalcoholic Extract in Cyclophosphamide-Induced Immunosuppression in Rats. Foods 12 (19). doi: https://doi.org/10.3390/foods12193652
- Pereira GA, Chaves DSA, Silva TME, Motta REA, Silva A, Patricio T, Fernandes AJB, Coelho SMO, Ozarowski M, Cid YP, Karpinski TM (2023) Antimicrobial Activity of Psidium guajava Aqueous Extract against Sensitive and Resistant Bacterial Strains. Microorganisms 11 (7). doi: https://doi.org/10.3390/microorganisms11071784
- Zheng M, Chen S, Liu Y, He Y (2023) alpha-Glucosidase inhibitory activities of constituents from Psidium guajava leaves. Nat Prod Res: 1-4. doi: https://doi.org/10.1080/14786419.2023.2238113
- Hu D, Fumoto S, Miyamoto H, Tanaka M, Nishida K (2022) Flavonoids Enhance Lipofection Efficiency and Ameliorate Cytotoxicity in Colon26 and HepG2 Cells via Oxidative Stress Regulation. Pharmaceutics 14 (6). doi: https://doi.org/10.3390/pharmaceutics14061203
- Ruksiriwanich W, Khantham C, Muangsanguan A, Phimolsiripol Y, Barba FJ, Sringarm K, Rachtanapun P, Jantanasakulwong K, Jantrawut P, Chittasupho C, Chutoprapat R, Boonpisuttinant K, Sommano SR (2022) Guava (Psidium guajava L.) Leaf Extract as Bioactive Substances for Anti-Androgen and Antioxidant Activities. Plants (Basel) 11 (24). doi: https://doi.org/10.3390/plants11243514
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