Document Type : Original Article


1 Department of Zoology, Bacha Khan University, Charsadda 24540, Khyber Pakhtunkhwa, Pakistan

2 Department of Chemistry, Bacha Khan University, Charsadda 24540, Khyber Pakhtunkhwa, Pakistan


In Pakistan, Urinary tract infections (UTI) are increasing day by day. The study therefore was designed for isolation identification and antibiotic resistance assessment of UTI causing bacteria. Bacterial pathogens causing UTI in dogs and human are becoming more resistant to antibiotic use. To evaluate the diversity and antibiotic resistance of uropathogenic bacteria, a total of 80 urine samples were collected in sterile containers. A total of 15 urine samples were taken from each gender of human and dogs with UTIs and 5 from each gender of healthy human and dogs. Samples were cultured for isolation and confirmed by biochemical tests and their antibiotic resistance pattern was checked by Kirby baur disk diffusion test. Samples that were taken from UTI males, E.coli, Proteus spp., Klebsiella spp. and Staph aureus was isolated from 93%, 6.67%,20% and 53% processed samples whereas from UTI females E.coli, Proteus spp., klebsiella spp., and Staph aureus was isolated 100%,13%,33%and 40% of samples respectively. Similarly 60% Staph aureus was isolated from healthy males and females urine samples. While Samples that were taken from UTI dogs E.coli., Proteus spp., Klebsiella spp., and Staph aureus was isolated from 66.7%, 6.67%, 33% and 26.6% of processed samples whereas Samples that were taken from bitches, E.coli., Proteus spp., klebsiella spp., and Staph aureus was isolated from 73%,13%,26.6% and 26.6% of samples respectively. In case of healthy dogs and bitches urine smalpes 60% and 80% of Staph aureus was isolated. Antibiotic resistance pattern of isolates E.coli, Proteus spp., klebsiella spp. and Staph aureus from human (male and female) samples showed resistance to ceftriaxone, Levofloxecine, and mild type of resistance to ofloxacin while sensitive to ciprofloxacin and sulfamethazole.The antibiotic resistance pattern of isolates E.coli, Proteus spp., Klebsiella spp. and Staph aureus from dogs and bitches samples showed high resistance to Lincomycin and kanamycin, and lower resistance to norfloxacin and doxycyclin while sensitive to ciprofloxacin, amoxaclin.

Graphical Abstract

Diversity and antibiotic resistance profile analysis of uropathogenic bacteria in human and canines


Main Subjects

Selected author of this article by journal

Shah Mansoor
Bacha Khan University


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  1. Dinh A, Duran C, Hamami K, Afif M, Bonnet F, Donay JL, Lafaurie M, Chartier-Kastler E (2022) Hyaluronic Acid and Chondroitin Sulphate Treatment for Recurrent Severe Urinary Tract Infections due to Multidrug-Resistant Gram-Negative Bacilli in a Patient With Multiple Sclerosis: Case Report and Literature Review. Open forum infectious diseases 9 (7): ofac245. doi:
  2. Liu H, Li Z, Shen R, Li Z, Yang Y, Yuan Q (2021) Point-of-Care Pathogen Testing Using Photonic Crystals and Machine Vision for Diagnosis of Urinary Tract Infections. Nano letters 21 (7): 2854-2860. doi:
  3. Schappert SM, Rechtsteiner EA (2011) Ambulatory medical care utilization estimates for 2007. Vital and Health Statistics Series 13, Data from the National Health Survey 169 (169): 1-38. doi:
  4. Foxman B (2014) Urinary tract infection syndromes: occurrence, recurrence, bacteriology, risk factors, and disease burden. Infectious Disease Clinics 28 (1): 1-13. doi:
  5. Davies E, Jolles D (2022) Safe prevention of Clostridium difficile using infectious disease guidelines at an urban hospital in North Carolina. BMJ open quality 11 (4). doi:
  6. Mitchell M, Nguyen SV, Macori G, Bolton D, McMullan G, Drudy D, Fanning S (2022) Clostridioides difficile as a Potential Pathogen of Importance to One Health: A Review. Foodborne Pathog Dis 19 (12): 806-816. doi:
  7. Serena TJ, Antypas E, Malay N, Laveroni E, Jr. (2020) Endovascular Intervention of a Mycotic Pseudoaneurysm of Accessory Left Hepatic Artery Arising from the Left Gastric Artery Presenting Secondary to Clostridium difficile Colitis: A Case Report. Cureus 12 (4): e7802. doi:
  8. Tibesar E (2020) Clostridium difficile Infection in a Very Young Infant with Pseudomembranous Colitis Noted on Endoscopy. Case reports in gastroenterology 14 (3): 522-526. doi:
  9. Hooton TM (2012) Uncomplicated urinary tract infection. New England Journal of Medicine 366 (11): 1028-1037. doi:
  10. Hannan TJ, Totsika M, Mansfield KJ, Moore KH, Schembri MA, Hultgren SJ (2012) Host–pathogen checkpoints and population bottlenecks in persistent and intracellular uropathogenic Escherichia coli bladder infection. FEMS microbiology reviews 36 (3): 616-648. doi:
  11. Huang R, Yuan Q, Gao J, Liu Y, Jin X, Tang L, Cao Y (2023) Application of metagenomic next-generation sequencing in the diagnosis of urinary tract infection in patients undergoing cutaneous ureterostomy. Front Cell Infect Microbiol 13: 991011. doi:
  12. Zhou Y, Zhou Z, Zheng L, Gong Z, Li Y, Jin Y, Huang Y, Chi M (2023) Urinary Tract Infections Caused by Uropathogenic Escherichia coli: Mechanisms of Infection and Treatment Options. Int J Mol Sci 24 (13). doi:
  13. Bjorling DE, Wang ZY, Bushman W (2011) Models of inflammation of the lower urinary tract. Neurourology and urodynamics 30 (5): 673-682. doi:
  14. Stamm WE, Norrby SR (2001) Urinary tract infections: disease panorama and challenges. The Journal of infectious diseases 183 (Supplement_1): S1-S4. doi:
  15. Ylhainen A, Molsa S, Gronthal T, Junnila J, Rantala M, Laitinen-Vapaavuori O, Thomson K (2023) A double-blinded randomized placebo-controlled non-inferiority trial protocol for postoperative infections associated with canine pyometra. BMC Vet Res 19 (1): 77. doi:
  16. Ayesha BB, Gachinmath S, Sobia C (2022) Isolation of obligate anaerobes from clinical samples received for routine bacterial culture and sensitivity: a cross sectional study. Iran J Microbiol 14 (2): 145-155. doi:
  17. Bazaid AS, Saeed A, Alrashidi A, Alrashidi A, Alshaghdali K, S AH, Alreshidi T, Alshammary M, Alarfaj A, Thallab R, Aldarhami A (2021) Antimicrobial Surveillance for Bacterial Uropathogens in Ha'il, Saudi Arabia: A Five-Year Multicenter Retrospective Study. Infect Drug Resist 14: 1455-1465. doi:
  18. Navarro S, Sherman E, Colmer-Hamood JA, Nelius T, Myntti M, Hamood AN (2022) Urinary Catheters Coated with a Novel Biofilm Preventative Agent Inhibit Biofilm Development by Diverse Bacterial Uropathogens. Antibiotics (Basel, Switzerland) 11 (11). doi:
  19. Chan OSK, Baranger-Ete M, Lam WWT, Wu P, Yeung M, Lee E, Bond H, Swan O, Tun HM (2022) A Retrospective Study of Antimicrobial Resistant Bacteria Associated with Feline and Canine Urinary Tract Infection in Hong Kong SAR, China-A Case Study on Implication of First-Line Antibiotics Use. Antibiotics (Basel, Switzerland) 11 (9). doi:
  20. Dablool AS (2023) An Antibiogram Study for Urine Culture Testing in Makkah Region Hospitals. Cureus 15 (3): e36012. doi:
  21. Sayiner HS, Yilmazer MI, Abdelsalam AT, Ganim MA, Baloglu C, Altunoglu YC, Gur M, Saracoglu M, Attia MS, Mahmoud SA, Mohamed EH, Boukherroub R, Al-Shaalan NH, Alharthi S, Kandemirli F, Amin MA (2022) Synthesis and characterization of new 1,3,4-thiadiazole derivatives: study of their antibacterial activity and CT-DNA binding. RSC Adv 12 (46): 29627-29639. doi:
  22. Hsueh P-R, Ko W-C, Wu J-J, Lu J-J, Wang F-D, Wu H-Y, Wu T-L, Teng L-J (2010) Consensus statement on the adherence to Clinical and Laboratory Standards Institute (CLSI) Antimicrobial Susceptibility Testing Guidelines (CLSI-2010 and CLSI-2010-update) for Enterobacteriaceae in clinical microbiology laboratories in Taiwan. Journal of Microbiology, Immunology and Infection 43 (5): 452-455. doi:
  23. Maisto M, Iannuzzo F, Novellino E, Schiano E, Piccolo V, Tenore GC (2023) Natural Polyphenols for Prevention and Treatment of Urinary Tract Infections. Int J Mol Sci 24 (4). doi:
  24. Codelia-Anjum A, Lerner LB, Elterman D, Zorn KC, Bhojani N, Chughtai B (2023) Enterococcal Urinary Tract Infections: A Review of the Pathogenicity, Epidemiology, and Treatment. Antibiotics (Basel, Switzerland) 12 (4). doi:
  25. Berhe DF, Beyene GT, Seyoum B, Gebre M, Haile K, Tsegaye M, Boltena MT, Tesema E, Kibret TC, Biru M, Siraj DS, Shirley D, Howe R, Abdissa A (2021) Prevalence of antimicrobial resistance and its clinical implications in Ethiopia: a systematic review. Antimicrobial Resistance & Infection Control 10 (1): 168. doi:
  26. Beyene G, Tsegaye W (2011) Bacterial uropathogens in urinary tract infection and antibiotic susceptibility pattern in jimma university specialized hospital, southwest ethiopia. Ethiopian journal of health sciences 21 (2): 141-146. doi:
  27. Humayun T, Iqbal A (2012) The culture and sensitivity pattern of urinary tract infections in females of reproductive age group. Ann Pak Inst Med Sci 8 (1): 19-22. doi:
  28. Lakoh S, Maruta A, Kallon C, Deen GF, Russell JBW, Fofanah BD, Kamara IF, Kanu JS, Kamara D, Molleh B, Adekanmbi O, Tavernor S, Guth J, Sagili KD, Wilkinson E (2022) How Well Are Hand Hygiene Practices and Promotion Implemented in Sierra Leone? A Cross-Sectional Study in 13 Public Hospitals. Int J Environ Res Public Health 19 (7). doi:
  29. Liu J, Zhang W, Liu Y, Zhu W, Yuan Z, Su X, Ding C (2023) Differences in phyllosphere microbiomes among different Populus spp. in the same habitat. Front Plant Sci 14: 1143878. doi:
  30. Stothart MR, Newman AEM (2021) Shades of grey: host phenotype dependent effect of urbanization on the bacterial microbiome of a wild mammal. Animal microbiome 3 (1): 46. doi:
  31. Al Zuhairi JJMJ, Jookar Kashi F, Rahimi-Moghaddam A, Yazdani M (2020) Antioxidant, cytotoxic and antibacterial activity of Rosmarinus officinalis L. essential oil against bacteria isolated from urinary tract infection. European Journal of Integrative Medicine 38: 101192. doi:
  32. Zhao F, Yang H, Bi D, Khaledi A, Qiao M (2020) A systematic review and meta-analysis of antibiotic resistance patterns, and the correlation between biofilm formation with virulence factors in uropathogenic E. coli isolated from urinary tract infections. Microbial Pathogenesis 144: 104196. doi:
  33. Ball KR, Rubin JE, Chirino-Trejo M, Dowling PM (2008) Antimicrobial resistance and prevalence of canine uropathogens at the Western College of Veterinary Medicine Veterinary Teaching Hospital, 2002–2007. The Canadian Veterinary Journal 49 (10): 985-990. doi:
  34. Çetin C, ┼×ENTÜRK S, Kocabiyik AL, Temizel M, Özel E (2003) Bacteriological examination of urine samples from dogs with symptoms of urinary tract infection. Turkish Journal of Veterinary & Animal Sciences 27 (5): 1225-1229. doi:
  35. Gezmu T, Regassa B, Manilal A, Mama M, Merdekios B (2016) Prevalence, diversity and antimicrobial resistance of bacteria isolated from the UTI patients of Arba Minch Province, southern Ethiopia. Transl Biomed 7 (3): 81. doi:
  36. Papini R, Ebani VV, Cerri D, Guidi G (2006) Survey on bacterial isolates from dogs with urinary tract infections and their in vitro sensitivity. Revue de médecine vétérinaire 157 (1): 35-41. doi:
  37. Tuniyazi M, Hu X, Fu Y, Zhang N (2022) Canine Fecal Microbiota Transplantation: Current Application and Possible Mechanisms. Veterinary Sciences 9 (8): 396. doi:
  38. Braný D, Dvorská D, Halašová E, Škovierová H (2020) Cold Atmospheric Plasma: A Powerful Tool for Modern Medicine. International Journal of Molecular Sciences 21 (8): 2932. doi:
  39. Sim JJ, Lau SF, Omar S, Watanabe M, Aslam MW (2021) A Retrospective Study on Bacteriology, Clinicopathologic and Radiographic Features in 28 Cats Diagnosed with Pyothorax. Animals 11 (8): 2286. doi: