Original Article
Bioinformatics
Ali Reza Mirzaei; Farzaneh Fazeli
Abstract
Autophagy is an effective regulatory process for eliminating tumors and worn-out intracellular components. Different groups of enzymes and regulatory elements are involved in the autophagy process. MAP1LC3A and BECN1 genes are the most important gene groups in autophagy. These genes, through the production ...
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Autophagy is an effective regulatory process for eliminating tumors and worn-out intracellular components. Different groups of enzymes and regulatory elements are involved in the autophagy process. MAP1LC3A and BECN1 genes are the most important gene groups in autophagy. These genes, through the production of beclin-1 and lc3 proteins, are involved in the production of autophagosomes. In general, both MAP1LC3A and BECN1 genes are active in cellular responses and the biological process. The aim of this study was bioinformatics analysis at the level of genome and proteome and to evaluate and compare the expression of MAP1LC3A and BECN1 genes in different human body tissues. The results of this study showed that the expression level of the BECN1 gene was relatively higher than the MAP1LC3A gene in different mammals. Cell analysis of MAP1LC3A and BECN1 genes by antibodies that bind to proteins of target genes showed that the protein encoded by the BECN1 gene is more present in the cytosol and the proteins encoded by MAP1LC3A gene are locally present in vesicles. It was also found that the protein encoded by the MAP1LC3A gene had a higher expression in brain tissues than in other tissues, while the beclin-1 protein in cardiac tissue showed higher expression than in other tissues. Finally, by using this information, it is possible to provide the ground for targeted therapies.
Review Article
Medical
Mohammad Reza Mohammadi; Amir Hossein Omidi; Hoda Sabati
Abstract
SARS-CoV- 2 is the causative agent of the global pandemic, also known as Covid-19. This virus belongs to a group of coronaviruses and has affected more than ten million people across the globe, causing nearly half a million deaths worldwide. The pandemic has spread worldwide, originating in the Wuhan ...
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SARS-CoV- 2 is the causative agent of the global pandemic, also known as Covid-19. This virus belongs to a group of coronaviruses and has affected more than ten million people across the globe, causing nearly half a million deaths worldwide. The pandemic has spread worldwide, originating in the Wuhan Hubei province of China in 2019. The disease is a significant challenge as there is no antiviral treatment. This review will address current trends and emerging new methods for detecting SARS-CoV-2 in the laboratoryat present. Reverse transcriptase PCR or RT-PCR is the gold standard for detecting SARS-CoV-2 disease. The seroprevalence of Covid-19 is performed using antibody detection tests using ELISA and antigen detection as rapid tests. In clinical practice, preliminary disease identification is made based on Chest radiographs, computed tomography, and positron emission tomography (PET) scans. As the pandemic has progressed, newer methods of detection like CRISPR, nanotechnology-enabled solutions, and biosensors have emerged as new methods of detecting SARS-CoV-2.
Original Article
Medical
I Nyoman Arsana; Ni Ketut Ayu Juliasih; A A Ayu Sauca Sunia Widyantari; Ni Luh Suriani; Agus Manto
Abstract
Pepper (Piper nigrum L.) has been utilized since antiquity and is considered the king of spices due to its wide use. This study aims to analyze the active compounds of white pepper through GC-MS and their pharmacological effects. Pepper seeds were extracted using 96% ethanol as solvent by the maceration ...
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Pepper (Piper nigrum L.) has been utilized since antiquity and is considered the king of spices due to its wide use. This study aims to analyze the active compounds of white pepper through GC-MS and their pharmacological effects. Pepper seeds were extracted using 96% ethanol as solvent by the maceration method. The active compounds in the dry extract were then analyzed by GC-MS. Identification of the active compound was carried out by matching it to the Willey7 Library database. Based on the results chromatogram, is known that there are 127 components of the compound, of which there are 11 main components. Most of the main components are alkaloids and have various pharmacological effects discussed. Piperidine, Caryophyllene, and Ethyl iso-allocholate are some of active compounds in ethanol extract. Ethyl iso-allocholate acts as an anti-inflammatory with a strong affinity for the target protein and also acts as an antiviral for SARS-CoV by inhibiting the attachment of the viral genome to target proteins, namely angiotensin-converting enzyme 2 (ACE2) and main protease (MPro).
Original Article
Gene Expression Studies
Seyyedeh Shabnam Irankhah; Saied Hoseini-Asl; Mehdi Valizadeh; Firouz Amani
Abstract
Background: Beta-thalassemia is one of the most common genetic diseases with autosomal recessive inherited patterns in the world and is one of the most common diseases in Iran that exists in all age and sex groups. Determining gene mutations in this disease can be effective in controlling and treating ...
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Background: Beta-thalassemia is one of the most common genetic diseases with autosomal recessive inherited patterns in the world and is one of the most common diseases in Iran that exists in all age and sex groups. Determining gene mutations in this disease can be effective in controlling and treating the disease. The present study determined the frequency of polymorphisms of Hinc, RSaI, RDB, and Xmn in patients with beta-thalassemia minor in Ardabil province. Methods: 53 beta-thalassemia patients referred to the genetic department of Imam Khomeini Hospital were studied. Blood samples were taken to determine the type of gene mutation. PCR samples were genetically evaluated to determine genetic mutations using RDB-Sequence-RFLP-Haplotype methods. Results: A total of 53 samples were examined, of which 56.6% were male and the rest were female. The most positive cases in the first and second ranks were related to XmnI and AvaII enzymes with 73.5% and 60.3%, respectively. The most common mutation extracted in the studied samples with 14 cases (26.4%) was IVS2.1. Among the most common mutations extracted by the RDB method was related to IVS 1.2 with 26.4%. Conclusions: The results of the present study showed that the distribution of genetic mutations in the studied samples can be different from other places. Also, by performing targeted genetic counseling, it is possible to control and prevent the disease in the future.
Review Article
Biochemistry
Umesh Pravin Dhuldhaj; Namrata Malik
Abstract
Soil microbial flora has a pivotal role in the phyto-availability of phosphorus and other necessary minerals and nutrients. The primary class of Rhizobacteria involved in the solubilization of phosphate from non-available forms to available forms is Phosphate solubilizing bacteria (PSB). The application ...
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Soil microbial flora has a pivotal role in the phyto-availability of phosphorus and other necessary minerals and nutrients. The primary class of Rhizobacteria involved in the solubilization of phosphate from non-available forms to available forms is Phosphate solubilizing bacteria (PSB). The application of Phosphate solubilizing bacteria increased phosphorus availability, which is one of the major factors responsible for the increase in the yield of crops. The phosphorus content is higher in the seeds than in the other plant parts; it helps plants in disease resistance and stress management such as winter rigors and improves the quality of fruits, vegetables, and cereal crops. Application of PSB as the biofertilizers positively affects the secretion of siderophores, nitrogen fixation, Indole acetic acid (IAA), 1-aminocylopropane-1-carboxylate (ACC) deaminase, chitinase, and protease. PSB can solubilize useful phosphate from rock phosphate and phosphate present in the combined state in lower to higher pH range (4 to 10), lower to a higher temperature (20 to 40 0C), and even in the higher salt ranges (0 to 7.5 % NaCl). Microbes help in the assimilation of phosphates and hydrocarbons by the secretions of different phosphatases such as monoesterase, diesterases, C-P lyase, and phosphatase and phytases. Using chemical P fertilizer in sustainable agricultural methods needs to be reduced. For this purpose, alternative and inexpensive technology are required so that plants can be provided with a sufficient amount of P. Phosphate solubilizing microbes can be an excellent option to replace chemical P fertilizers for improved agricultural production and soil fertility. The fertility of farm fields can be improved by using PSB as the bio-fertilizer and it will enhance the nutritional quality of plants and plant products which are directly or indirectly taken as food. Applying these microbes to soil/seeds makes good quality fruits and can help to fulfill the nutritional hunger of the world.
Original Article
Application of medicinal plants in medicine
Shagoon Tabin; Raghubir Chand Gupta; Azra Nahid kamili; Javid Ahmad parray
Abstract
Most of the plants are essential due to their medicinal properties and have contributed to the origin and evolution of many traditional herbal therapies. Rheum is also one of the famous and known medicinal plants of Kashmir Himalaya. It ranges from an altitude of 1700-5500 m. Its Roots are long and stout. ...
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Most of the plants are essential due to their medicinal properties and have contributed to the origin and evolution of many traditional herbal therapies. Rheum is also one of the famous and known medicinal plants of Kashmir Himalaya. It ranges from an altitude of 1700-5500 m. Its Roots are long and stout. The stem is erect, hollow, sulcate, glabrous, or strigose. It is native to Asia-Temperate to Asia-Tropical, from China to India, Nepal, and Pakistan. In India, its found in Himachal Pradesh, Jammu and Kashmir, and Uttar Pradesh. Rhubarb is the English name of the genus Rheum (family Polygonaceae). Emodi, Webbianum and Spiciforme are three species of Rheum found in Kashmir Himalaya. Rheum species are well-known medicinal plant and is found on high altitudes. Its leaves and stalks are taken as food and cooked as vegetables. It’s used as medicine and is generally proven more effective against certain ailments like antibacterial, anticancerous, anti-inflammatory, rheumatoid diseases, stomachache, and intestinal problems. It is also used for the treatment of indigestion, abdominal disorders, boils, wounds and flatulence. But Rheum has become endangered due to exploitation. Active compounds like aloe-emodin, rhein, emodin, chrysaphanol and physicion are found in this plant which is anti Cancerian agents. High molecular diversity is also observed in all the three species of Rheum.
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