Document Type : Original Article
1 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 Department of biology, Payame Noor University (PNU), P.O.Box, 19395-4697 Terhran, Iran
Autophagy is an effective regulatory process for eliminating tumors and worn-out intracellular components. Different groups of enzymes and regulatory elements are involved in the autophagy process. MAP1LC3A and BECN1 genes are the most important gene groups in autophagy. These genes, through the production of beclin-1 and lc3 proteins, are involved in the production of autophagosomes. In general, both MAP1LC3A and BECN1 genes are active in cellular responses and the biological process. The aim of this study was bioinformatics analysis at the level of genome and proteome and to evaluate and compare the expression of MAP1LC3A and BECN1 genes in different human body tissues. The results of this study showed that the expression level of the BECN1 gene was relatively higher than the MAP1LC3A gene in different mammals. Cell analysis of MAP1LC3A and BECN1 genes by antibodies that bind to proteins of target genes showed that the protein encoded by the BECN1 gene is more present in the cytosol and the proteins encoded by MAP1LC3A gene are locally present in vesicles. It was also found that the protein encoded by the MAP1LC3A gene had a higher expression in brain tissues than in other tissues, while the beclin-1 protein in cardiac tissue showed higher expression than in other tissues. Finally, by using this information, it is possible to provide the ground for targeted therapies.
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- Fazeli-Nasab B, Sayyed RZ, Sobhanizadeh A (2021) In Silico Molecular Docking Analysis of α-Pinene: An Antioxidant and Anticancer Drug Obtained from Myrtus communis. Int J Cancer Manag 14(2):e89116. doi:https://doi.org/10.5812/ijcm.89116
- Kabeya Y, Mizushima N, Yamamoto A, Oshitani-Okamoto S, Ohsumi Y, Yoshimori T (2004) LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation. Journal of cell science 117(13):2805-2812. doi:https://doi.org/10.1242/jcs.01131
- Lisiak N, Toton E, Rybczynska M (2018) Autophagy as a potential therapeutic target in breast cancer treatment. Current Cancer Drug Targets 18(7):629-639. doi:https://doi.org/10.2174/1568009617666171114143330
- Parzych KR, Klionsky DJ (2014) An overview of autophagy: morphology, mechanism, and regulation. Antioxidants & redox signaling 20(3):460-473. doi:https://doi.org/10.1089/ars.2013.5371
- Nabavi SF, Sureda A, Dehpour AR, Shirooie S, Silva AS, Devi KP, Ahmed T, Ishaq N, Hashim R, Sobarzo-Sánchez E (2018) Regulation of autophagy by polyphenols: Paving the road for treatment of neurodegeneration. Biotechnology advances 36(6):1768-1778. doi:https://doi.org/10.1016/j.biotechadv.2017.12.001
- Sharma R, Yadav R, Manivannan E (2012) Study of effect of Stevia rebaudiana bertoni on oxidative stress in type-2 diabetic rat models. Biomedicine & Aging Pathology 2(3):126-131. doi:https://doi.org/10.1016/j.biomag.2012.07.001
- Xu H-D, Qin Z-H (2019) Beclin 1, Bcl-2 and Autophagy. In: Qin Z-H (ed) Autophagy: Biology and Diseases: Basic Science. Springer Singapore, Singapore, pp 109-126. doi:https://doi.org/10.1007/978-981-15-0602-4_5
- Wijshake T, Zou Z, Chen B, Zhong L, Xiao G, Xie Y, Doench JG, Bennett L, Levine B (2021) Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin. Proceedings of the National Academy of Sciences 118(5):e2020478118. doi:https://doi.org/10.1073/pnas.2020478118
- Orvedahl A, MacPherson S, Sumpter Jr R, Tallóczy Z, Zou Z, Levine B (2010) Autophagy protects against Sindbis virus infection of the central nervous system. Cell host & microbe 7(2):115-127. doi:https://doi.org/10.1016/j.chom.2010.01.007
- Ma X, Liu H, Murphy JT, Foyil SR, Godar RJ, Abuirqeba H, Weinheimer CJ, Barger PM, Diwan A (2015) Regulation of the transcription factor EB-PGC1α axis by beclin-1 controls mitochondrial quality and cardiomyocyte death under stress. Molecular and cellular biology 35(6):956-976. doi:https://doi.org/10.1128/MCB.01091-14
- Kang Y, Li Y, Zhang T, Chi Y, Liu M (2019) Effects of transcription factor EB on oxidative stress and apoptosis induced by high glucose in podocytes. International journal of molecular medicine 44(2):447-456. doi:https://doi.org/10.3892/ijmm.2019.4209
- Fink G, Szewczak-Harris A, Löwe J (2016) SnapShot: the bacterial cytoskeleton. Cell 166(2):522-522. e521. doi:http://dx.doi.org/10.1016/j.cell.2016.06.057
- Grant BD, Donaldson JG (2009) Pathways and mechanisms of endocytic recycling. Nature reviews Molecular cell biology 10(9):597-608. doi:https://doi.org/10.1038/nrm2755
- Ziółkowska B, Woźniak M, Ziółkowski P (2016) Co-expression of autophagic markers following photodynamic therapy in SW620 human colon adenocarcinoma cells. Molecular medicine reports 14(3):2548-2554. doi:https://doi.org/10.3892/mmr.2016.5541
- Zhou X, Wong ST (2008) Computational systems bioinformatics and bioimaging for pathway analysis and drug screening. Proceedings of the IEEE 96(8):1310-1331. doi:https://doi.org/10.1109/JPROC.2008.925440
- Martin DD, Ladha S, Ehrnhoefer DE, Hayden MR (2015) Autophagy in Huntington disease and huntingtin in autophagy. Trends in neurosciences 38(1):26-35. doi:https://doi.org/10.1016/j.tins.2014.09.003
- Sharma M, Rajendrarao S, Shahani N, Ramírez-Jarquín UN, Subramaniam S (2020) Cyclic GMP-AMP synthase promotes the inflammatory and autophagy responses in Huntington disease. Proceedings of the National Academy of Sciences 117(27):15989-15999. doi:https://doi.org/10.1073/pnas.2002144117
- Giatromanolaki AN, St Charitoudis G, Bechrakis NE, Kozobolis VP, Koukourakis MI, Foerster MH, Sivridis EL (2011) Autophagy patterns and prognosis in uveal melanomas. Modern Pathology 24(8):1036-1045. doi:https://doi.org/10.1038/modpathol.2011.63
- Paul-Samojedny M, Pudełko A, Kowalczyk M, Fila-Daniłow A, Suchanek-Raif R, Borkowska P, Kowalski J (2015) Knockdown of AKT3 and PI3KCA by RNA interference changes the expression of the genes that are related to apoptosis and autophagy in T98G glioblastoma multiforme cells. Pharmacological Reports 67(6):1115-1123. doi:https://doi.org/10.1016/j.pharep.2015.04.012
- He H, Dang Y, Dai F, Guo Z, Wu J, She X, Pei Y, Chen Y, Ling W, Wu C (2003) Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B. Journal of biological chemistry 278(31):29278-29287. doi:https://doi.org/10.1074/jbc.M303800200
- Liang XH, Kleeman LK, Jiang HH, Gordon G, Goldman JE, Berry G, Herman B, Levine B (1998) Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein. Journal of virology 72(11):8586-8596
- Liang X Jackson S, Seaman M, Brown K, Kempkes B, Hibshoosh H, Levine B. Induction of autophagy and inhibition of tumorigenesis by beclin 1:672-676
- Mutlu H, Mutlu S, Bostancıklıoğlu M (2021) Profiling of autophagy-associated microRNAs in the osteosarcoma cell line of U2OS. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents) 21(13):1732-1737. doi:https://doi.org/10.2174/1871520621666201202090128