Document Type : Original Article
1 Medical Laboratory Technique Department, The Islamic University, Diwaniya, Iraq
2 Research and Studies Department, The Islamic University, Najaf, Iraq
Emerging antibiotic resistance in microorganisms particularly multidrug-resistant strains among bacteria are increasing because of misusing antibiotics as well as evolution of antibiotic resistance mechanisms. In this regard, Acinetobacter baumannii is one of the six most common multidrug-resistant microorganisms in hospitals. Recently, carbapenems, as common antibiotics to treat infections of Acinetobacter have not an acceptable efficiency because of the resistance emergence to these antibiotics in many strains. In this study, resistant strains of A. baumannii were isolated and identified as an appropriate preventive strategy to reduce infections in hospitals. Disc diffusion test and PCR method were used to isolate of resistant strains and identify beta-lactamase genes of blaAmpC, blaTEM, blaVIM, and blaSHV. This study showed that these genes were contributed in antibiotic resistance with about 18.4% and ≥53.5% strains expressing all 4 genes and ≥3 genes, respectively. The blaAmpC gene is more prevalent than other genes, and this is probably due to the prevalence or rapid transfer of this beta-lactamase. However, more studies should be performed in a comparative way to isolate and identify other antibiotic-resistant bacterial strains relate to other hospitals.
- nosocomial infections, antibiotic-resistant bacteria, carbapenems
- antibacterial activity, beta-lactamase genes, the blaAmpC gene
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- Ercisli MF, Lechun G, Azeez SH, Hamasalih RM, Song S, Aziziaram Z (2021) Relevance of genetic polymorphisms of the human cytochrome P450 3A4 in rivaroxaban-treated patients. Cell Mol Biomed Rep 1(1):33-41
- Tourang M, Fang L, Zhong Y, Suthar RC (2021) Association between Human Endogenous Retrovirus K gene expression and breast cancer. Cell Mol Biomed Rep 1(1):7-13
- Alavi M, Webster TJ (2021) Recent progress and challenges for polymeric microsphere compared to nanosphere drug release systems: Is there a real difference? Bioorg Med Chem 33:116028. doi:https://doi.org/10.1016/j.bmc.2021.116028
- Aljelehawy Q, Karimi N, Alavi M (2020) Comparison of antibacterial and cytotoxic activities of phytosynthesized ZnONPs by leaves extract of Daphne mucronata at different salt sources. Mater Technol):1-13. doi:https://doi.org/10.1080/10667857.2020.1794280
- Li B, Webster TJ (2018) Bacteria antibiotic resistance: New challenges and opportunities for implant‐associated orthopedic infections. J Orthop Res 36(1):22-32. doi:http://doi.org/10.1002/jor.23656
- Alavi M, Rai M (2021) Chapter 11 - Antibacterial and wound healing activities of micro/nanocarriers based on carboxymethyl and quaternized chitosan derivatives. In: Rai M, dos Santos CA (eds) Biopolymer-Based Nano Films. Elsevier, pp 191-201. doi:https://doi.org/10.1016/B978-0-12-823381-8.00009-0
- Alavi M, Dehestaniathar S, Mohammadi S, Maleki A, Karimi N (2020) Antibacterial activities of phytofabricated ZnO and CuO NPs by Mentha pulegium leaf/flower mixture extract against antibiotic resistant bacteria. Adv Pharm Bull 1:1-14. doi:http://doi.org/10.34172/apb.2021.057
- Georgios M, Egki T, Effrosyni S (2014) Phenotypic and molecular methods for the detection of antibiotic resistance mechanisms in Gram negative nosocomial pathogens. Trends Infect Dis):139-162. doi:http://doi.org/10.5772/57582
- Ayoub Moubareck C, Hammoudi Halat D (2020) Insights into Acinetobacter baumannii: A Review of Microbiological, Virulence, and Resistance Traits in a Threatening Nosocomial Pathogen. Antibiotics 9(3):119
- Bergogne-Bérézin E, Joly-Guillou ML, Towner KJ (2020) Introduction—History and Importance of Acinetobacter spp., Role in Infections, Treatment and Cost Implications. In: Acinetobacter. CRC Press, pp 1-12
- Chan P-C, Huang L-M, Lin H-C, Chang L-Y, Chen M-L, Lu C-Y, Lee P-I, Chen J-M, Lee C-Y, Pan H-J (2007) Control of an outbreak of pandrug-resistant Acinetobacter baumannii colonization and infection in a neonatal intensive care unit. Infect Control Hosp Epidemiol 28(4):423-429. doi:http://doi.org/10.1086/513120
- Kyriakidis I, Vasileiou E, Pana ZD, Tragiannidis A (2021) Acinetobacter baumannii Antibiotic Resistance Mechanisms. Pathogens 10(3):373. doi:http://doi.org/10.3390/pathogens10030373
- Bush K, Jacoby GA (2010) Updated functional classification of beta-lactamases. Antimicrob Agents Chemother 54(3):969-976. doi:http://doi.org/10.1128/AAC.01009-09
- Lee C-R, Lee JH, Park M, Park KS, Bae IK, Kim YB, Cha C-J, Jeong BC, Lee SH (2017) Biology of Acinetobacter baumannii: pathogenesis, antibiotic resistance mechanisms, and prospective treatment options. Front Cell Infect Microbiol
- Paterson DL, Bonomo RA (2005) Extended-spectrum β-lactamases: a clinical update. Clin Microbiol Rev 18(4):657-686. doi:http://doi.org/10.1128/CMR.18.4.657-686.2005
- Alavi M, Karimi N (2019) Biosynthesis of Ag and Cu NPs by secondary metabolites of usnic acid and thymol with biological macromolecules aggregation and antibacterial activities against multi drug resistant (MDR) bacteria. Int J Biol Macromol 128:893-901. doi:https://doi.org/10.1016/j.ijbiomac.2019.01.177
- Piperaki ET, Tzouvelekis LS, Miriagou V, Daikos GL (2019) Carbapenem-resistant Acinetobacter baumannii: in pursuit of an effective treatment. Clin Microbiol Infect 25(8):951-957. doi:http://doi.org/10.1016/j.cmi.2019.03.014
- Leverstein-van Hall MA, Fluit AC, Paauw A, Box ATA, Brisse S, Verhoef J (2002) Evaluation of the Etest ESBL and the BD Phoenix, VITEK 1, and VITEK 2 automated instruments for detection of extended-spectrum beta-lactamases in multiresistant Escherichia coli and Klebsiella spp. J Clin Microbiol 40(10):3703-3711. doi:http://doi.org/10.1128/JCM.40.10.3703-3711.2002
- Afhami S, Borumand MA, Bazzaz NE, Saffar H, Hadadi A, Nezhad MJ, Tirabadi NM (2020) Antimicrobial resistance pattern of Acinetobacter; a multicenter study, comparing European Committee on Antimicrobial Susceptibility Testing (EUCAST) and the Clinical and Laboratory Standards Institute (CLSI); evaluation of susceptibility testing methods for polymyxin. Immunopathol persa 7(1):e04-e04. doi:http://doi.org/10.34172/ipp.2021.04
- Andrzejczuk S, Kosikowska U, Chwiejczak E, Stępień-Pyśniak D, Malm A (2019) Prevalence of resistance to β-lactam antibiotics and bla genes among commensal Haemophilus parainfluenzae isolates from respiratory microbiota in Poland. Microorganisms 7(10):427
- Ahmed OB, Dablool AS (2017) Quality improvement of the DNA extracted by boiling method in gram negative bacteria. Int J Bioassays 6(4):5347-5349. doi:http://dx.doi.org/10.21746/ijbio.2017.04.004
- Chang Y, Luan G, Xu Y, Wang Y, Shen M, Zhang C, Zheng W, Huang J, Yang J, Jia X (2015) Characterization of carbapenem-resistant Acinetobacter baumannii isolates in a Chinese teaching hospital. Front Microbiol 6:910. doi:http://doi.org/10.3389/fmicb.2015.00910
- Lowings M, Ehlers MM, Dreyer AW, Kock MM (2015) High prevalence of oxacillinases in clinical multidrug-resistant Acinetobacter baumannii isolates from the Tshwane region, South Africa–an update. BMC Infect Dis 15(1):1-10. doi:http://doi.org/10.1186/s12879-015-1246-8
- Yin X-L, Hou T-W, Xu S-B, Ma C-Q, Yao Z-Y, Li W, Wei L (2008) Detection of drug resistance–associated genes of multidrug-resistant Acinetobacter baumannii. Microb Drug Resist 14(2):145-150. doi:http://doi.org/10.1089/mdr.2008.0799
- Mohammed H, Elsadek Fakhr A, Al Johery SaE, Abdel Ghani Hassanein W (2016) Spread of TEM, VIM, SHV, and CTX-M β-lactamases in imipenem-resistant Gram-negative bacilli isolated from Egyptian hospitals. Int J Microbiol 2016. doi:http://doi.org/10.1155/2016/8382605
- Shali AAK (2012) Identification of Multidrug-Resistant Genes in" Acinetobacter baumannii" in Sulaimani City-Kurdistan Regional Government of Iraq. Asian J Med Sci 4(5):179-183
- Al-Hasnawy HH, Saleh RH, Hadi BH (2018) Existence ofaESBL genes in Escherichia coli and Acinetobacter baumannii isolated from different clinical specimens. J Pharm Sci 10(5):1112-1117. doi:https://www.jpsr.pharmainfo.in/issue.php?page=105#
- Aziz RAR, Al-Jubori SS (2017) Molecular analysis of genetic elements responsible for XDR in highly successful pathogen Acinetobacter baumannii isolated from clinical samples of Iraqi patients. J Glob Pharma Technol 9(4):26-39. doi:http://www.jgpt.co.in/index.php/jgpt/issue/archive?issuesPage=4#issues
- Al-Agamy MH, Khalaf NG, Tawfick MM, Shibl AM, El Kholy A (2014) Molecular characterization of carbapenem-insensitive Acinetobacter baumannii in Egypt. Int J Infect Dis 22:49-54. doi:https://doi.org/10.1016/j.ijid.2013.12.004
- Mushi MF, Mshana SE, Imirzalioglu C, Bwanga F (2014) Carbapenemase genes among multidrug resistant gram negative clinical isolates from a tertiary hospital in Mwanza, Tanzania. Biomed Res Int 2014. doi:https://doi.org/10.1155/2014/303104
- Hujer KM, Hujer AM, Hulten EA, Bajaksouzian S, Adams JM, Donskey CJ, Ecker DJ, Massire C, Eshoo MW, Sampath R (2006) Analysis of antibiotic resistance genes in multidrug-resistant Acinetobacter sp. isolates from military and civilian patients treated at the Walter Reed Army Medical Center. Antimicrob Agents Chemother 50(12):4114-4123. doi:http://doi.org/10.1128/AAC.00778-06
- Fallah F, Noori M, Hashemi A, Goudarzi H, Karimi A, Erfanimanesh S, Alimehr S (2014) Prevalence of blaNDM, blaPER, blaVEB, blaIMP, and blaVIM genes among Acinetobacter baumannii isolated from two hospitals of Tehran, Iran. Scientifica 2014. doi:http://doi.org/10.1155/2014/245162
- Al-Kadmy IMS, Ali ANM, Salman IMA, Khazaal SS (2018) Molecular characterization of Acinetobacter baumannii isolated from Iraqi hospital environment. New Microbes New Infect 21:51-57. doi:https://doi.org/10.1016/j.nmni.2017.10.010