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- Cellular, Molecular and Biomedical Reports Abbreviation: Cell. Mol. Biomed. Rep. CODEN: CMBR Cellular, Molecular a... moreCellular, Molecular and Biomedical Reports
Abbreviation: Cell. Mol. Biomed. Rep.
CODEN: CMBR
Cellular, Molecular and Biomedical Reports (CMBR) was launched by Global Sciences in 2021. This double peer-reviewed and indexed journal aim to provide a platform for researchers around the world to share their latest findings in all fields of Cellular, Molecular and Biomedical Reports. Experimental, theoretical and applied original research studies in all fields of chemistry are welcomed for submission. The journal is Open Access with article processing charges and all articles published in this journal are freely available for readers online at https://www.cmbr-journal.com/ without a subscription and authors retain the copyright of their work.
CMBR is published Semiannual (online and in print= 2 Issues per year).
All submitted manuscripts are checked for similarity through a trustworthy software named iThenticate to be assured about their originality and then rigorously peer-reviewed by international reviewers
Aims and Scope
Cellular, Molecular and Biomedical Reports is a scientific journal devoted entirely to all facets of pure and applied studies on all aspects of Cellular, Molecular and Biomedical sciences with emphasizing the latest techniques. Of course, The CMBR journal only accepts original, review, mini-review, or meta-analysis articles in the field of medicine or other organisms that have been used in medicine.
It is journal policy to publish work deemed by peer reviewers to be a coherent and sound addition to scientific knowledge and to put less emphasis on interest levels, provided that the research constitutes a useful contribution to the field.
Cellular, Molecular and Biomedical Reports focuses on:
- All aspects of Cellular, Molecular and Biomedical Reports
- All aspects related to the different structures and functions of the cell and to its components (DNA, RNA, protein) are relevant to the scope of this journal
- DNA replication, transcription, nucleic acid-protein interaction, RNA processing, intracellular transport, protein biosynthesis are examples of topics within the Cellular, Molecular and Biomedical field
- Nuclear function, cytoskeleton and cell membrane interactions, transport of cellular products between different organelles fits in the Cellular, Molecular and Biomedical category.
The CMBR journal invites the Researchers or Scientists, Research scholars, Physicians, Academician, Industrialists, Lab Technologists, PG Students, Consultancies, etc., to employ this journal for publishing articles for the development of Cellular, Molecular and Biomedical Sciences.edit
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Eight hundred and six newborn infants at high risk for glucose-6-phosphate dehydrogenase (G-6-PD) deficiency were screened; 30.2% of the boys and 10.4% of the girls had severe G-6-PD deficiency. Surprisingly, 14% of the enzyme deficient... more
Eight hundred and six newborn infants at high risk for glucose-6-phosphate dehydrogenase (G-6-PD) deficiency were screened; 30.2% of the boys and 10.4% of the girls had severe G-6-PD deficiency. Surprisingly, 14% of the enzyme deficient girls had a father from a low risk ethnic group. Girls of high risk mothers should be screened for G-6-PD deficiency regardless of paternal origin.
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Changes in blood sugar in poisoning can be one of the most important determinants of the outcome of patients with poisoning. Since poisoning is one of the most common and increasing causes of death worldwide and one of the most... more
Changes in blood sugar in poisoning can be one of the most important
determinants of the outcome of patients with poisoning. Since poisoning
is one of the most common and increasing causes of death worldwide
and one of the most critical medical emergencies, this study aimed to
investigate changes in blood sugar in patients with acute poisoning and
how patients' blood sugar can predict the severity and outcome of the
disease. The present study was performed on 200 patients with acute
drug poisoning referred to the emergency department of Amir Al-
Momenin Hospital in Zabol from March 2018 to March 2020. Blood
glucose levels of all patients were recorded at the time of admission and
every hour to the first 5 hours after admission, and the results were
entered in the information form of each patient. Finally, the data were
entered into SPSS V22 software and statistically analyzed. The mean age
of participants was 23.21 ± 12.80 years, and the minimum and maximum
age of patients were 1 year and 77 years, respectively, and only 9.8% of
them had a history of diabetes. In this study, the highest rate of
intoxication with opioids such as methadone and tramadol was (38%),
followed by benzodiazepines (20.5%), NSAIDs (19.5%), and SSRIs (7%)
were the most commonly used drugs. The prevalence of hypoglycemia in
this study was 3% (6 patients), while no cases of hyperglycemia were
reported. In this study, most changes in blood sugar were caused by
alcohol poisoning. Also, neuroleptics, NSAIDs, and chemicals had the
least changes in blood sugar. However, patients' blood sugar at the
beginning of poisoning did not affect patients' prognosis. The present
study results showed that changes in blood sugar during treatment
during drug intoxication, alcohol, medications(sulfonylurea,
glibenclamide), and NSAID are very important, so regular monitoring of
blood glucose in intoxication with these cases is essential
determinants of the outcome of patients with poisoning. Since poisoning
is one of the most common and increasing causes of death worldwide
and one of the most critical medical emergencies, this study aimed to
investigate changes in blood sugar in patients with acute poisoning and
how patients' blood sugar can predict the severity and outcome of the
disease. The present study was performed on 200 patients with acute
drug poisoning referred to the emergency department of Amir Al-
Momenin Hospital in Zabol from March 2018 to March 2020. Blood
glucose levels of all patients were recorded at the time of admission and
every hour to the first 5 hours after admission, and the results were
entered in the information form of each patient. Finally, the data were
entered into SPSS V22 software and statistically analyzed. The mean age
of participants was 23.21 ± 12.80 years, and the minimum and maximum
age of patients were 1 year and 77 years, respectively, and only 9.8% of
them had a history of diabetes. In this study, the highest rate of
intoxication with opioids such as methadone and tramadol was (38%),
followed by benzodiazepines (20.5%), NSAIDs (19.5%), and SSRIs (7%)
were the most commonly used drugs. The prevalence of hypoglycemia in
this study was 3% (6 patients), while no cases of hyperglycemia were
reported. In this study, most changes in blood sugar were caused by
alcohol poisoning. Also, neuroleptics, NSAIDs, and chemicals had the
least changes in blood sugar. However, patients' blood sugar at the
beginning of poisoning did not affect patients' prognosis. The present
study results showed that changes in blood sugar during treatment
during drug intoxication, alcohol, medications(sulfonylurea,
glibenclamide), and NSAID are very important, so regular monitoring of
blood glucose in intoxication with these cases is essential
Research Interests:
A type of viral pneumonia that broke out in Wuhan, China in December 2019, and new strains of it are spreading rapidly around the world, is called coronavirus disease 2019 (COVID-19). The incidence of cardiovascular complications has... more
A type of viral pneumonia that broke out in Wuhan, China in December 2019, and new strains of it are spreading rapidly around the world, is called coronavirus disease 2019 (COVID-19). The incidence of cardiovascular complications has increased both in the general population and in patients diagnosed with the disease during the COVID-19 epidemic. One of the conditions is stress cardiomyopathy, which may be related to COVID-19. During the COVID-19 epidemic, stress cardiomyopathy may be present as a complication of acute infection or as an indirect consequence of quarantine. These conditions have created complications in patient care that have led to increased adverse outcomes, hospitalization, and medical costs. Stress cardiomyopathy is caused by various emotional and physical stressors such as anger, sadness, happiness, surgery, multiple medications, general anesthesia, and infectious diseases. The outbreak of the new coronavirus has posed an ongoing challenge for these patients with several medical and economic consequences. Providing useful information on stress and COVID-19 to people with heart disease and their families can be very important, and also, education and psychotherapy of heart patients can reduce the number of hospitalization and their complications.
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Staphylococcus aureus contains numerous surface proteins called microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) that mediate binding to host tissues and initiate colonization leading to infection. Virulence... more
Staphylococcus aureus contains numerous surface proteins called microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) that mediate binding to host tissues and initiate colonization leading to infection. Virulence genes such as enzymes, toxins, adhesin proteins, cell surface proteins play an important role in the pathogenicity of S. aureus strains. The distribution and prevalence of virulence genes vary among S. aureus strains in different regions. However, the highest frequency of virulence genes among S. aureus strains is related to toxin genes. There are many PCR methods for detecting these microorganisms such as conventional PCR, multiplex PCR, reverse transcription PCR (RT-PCR), and quantitative PCR (qPCR). Therefore, this study aimed to investigate the presence of virulence genes among methicillin-resistant S. aureus (MRSA) strains. In this study, multiplex PCR technique was applied to determine the presence of virulence genes among MRSA strains. Results showed the frequency of virulence genes among bacterial strains isolated from Al-Najaf Al-Ashraf teaching hospital. In addition, among the strains, hla gene with 91% frequency, exhibited the highest prevalence among pathogenic genes. Sea, mecA, clfB, femA, fnbB, tsst, hlb genes with 88%, 65%, 54%, 45%, 39%, 27% and 13% were in the next ranks, respectively. This investigation showed mecA is a gene found in bacterial cells that allows them to be resistant to antibiotics such as methicillin and other penicillin-like antibiotics.
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The main purpose of microbial typing is to evaluate the relationships between microbial isolates. Microbial typing can use for identifying the source of infection by detecting a clonal link between the strains. Moreover, it can analyze... more
The main purpose of microbial typing is to evaluate the
relationships between microbial isolates. Microbial typing can use
for identifying the source of infection by detecting a clonal link
between the strains. Moreover, it can analyze outbreaks,
antimicrobial-resistant strains, and evaluate the effectiveness of
control measures so, the efficiency of monitoring systems would
increase. HAIs can affect hospitalized patients in all age ranges with
any clinical situation, and lead to death. Molecular epidemiology is
useful to determine genetic relatedness between isolated pathogens
from patients, and design proper prevention plans to prevent
infection through the hospital and community. Nowadays, typing
methods for a wide range of bacterial strains are known as essential
epidemiological tools to prevent and control infections in hospitals
and communities. Although basic typing methods were more
focused on phenotypic techniques like antibiogram and serotyping,
new methods are based on molecular techniques including PCR-
based methods and sequencing-based methods. Due to the high
frequency of methods, choosing the right one for research
applications seems difficult and requires basic knowledge about all
of them. In this review, we aim to introduce the most useful and
practical molecular typing techniques. Also, their utilization,
advantages, and disadvantages were compared
relationships between microbial isolates. Microbial typing can use
for identifying the source of infection by detecting a clonal link
between the strains. Moreover, it can analyze outbreaks,
antimicrobial-resistant strains, and evaluate the effectiveness of
control measures so, the efficiency of monitoring systems would
increase. HAIs can affect hospitalized patients in all age ranges with
any clinical situation, and lead to death. Molecular epidemiology is
useful to determine genetic relatedness between isolated pathogens
from patients, and design proper prevention plans to prevent
infection through the hospital and community. Nowadays, typing
methods for a wide range of bacterial strains are known as essential
epidemiological tools to prevent and control infections in hospitals
and communities. Although basic typing methods were more
focused on phenotypic techniques like antibiogram and serotyping,
new methods are based on molecular techniques including PCR-
based methods and sequencing-based methods. Due to the high
frequency of methods, choosing the right one for research
applications seems difficult and requires basic knowledge about all
of them. In this review, we aim to introduce the most useful and
practical molecular typing techniques. Also, their utilization,
advantages, and disadvantages were compared
Research Interests:
Emerging antibiotic resistance in microorganisms particularly multidrugresistant bacterial strains are increasing because of misusing antibiotics as well as the evolution of antibiotic resistance mechanisms in new strains. In this regard,... more
Emerging antibiotic resistance in microorganisms particularly multidrugresistant bacterial strains are increasing because of misusing antibiotics as well as the evolution of antibiotic resistance mechanisms in new strains. In this regard, Acinetobacter baumannii is one of the six most common multidrugresistant microorganisms related to nosocomial diseases. Recently, carbapenems, as common antibiotics to treat infections caused by Acinetobacter have not shown an acceptable efficiency because of the resistance emergence to carbapenems in many strains of this bacterium. In this study, resistant strains of A. baumannii were isolated and identified as an appropriate preventive strategy to reduce bacterial infections in al-yarmouk hospital of Iraq. Disc diffusion test and PCR method were used to isolate of resistant strains and identify beta-lactamase genes of blaAmpC, blaTEM, blaVIM, and blaSHV. This study showed that these genes were contributed to the antibiotic resistance with about 18.4% and ≥53.5% strains expressing all 4 genes and ≥3 genes, respectively. The blaAmpC gene is more prevalent than other genes, and this is probably due to the prevalence or rapid transfer of this beta-lactamase. However, more studies should be performed in a comparative way to isolate and identify other antibiotic-resistant bacterial strains associated with other hospitals. Article info
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Finding efficient therapeutic strategies to fight antibiotic-resistant bacteria is a complicated affair specifically in the therapy of chronic bacterial infections related to hospital-acquired infections. Recently, three major... more
Finding efficient therapeutic strategies to fight antibiotic-resistant bacteria is a complicated affair specifically in the therapy of chronic bacterial infections related to hospital-acquired infections. Recently, three major antibacterial systems based on antisense RNA, CRISPR-Cas9, and metal/metal oxide nanoparticles particularly silver (Ag) nanoparticles have shown more effective antibacterial activity compared to conventional antibiotics. ROS generation, attachment to the cell membrane, disruption of bacterial envelop, inactivation of electron transport chain, decreasing the local pH, modulation of cell signaling, and denaturation of biological macromolecules such as proteins and nucleic acids have been found as the main antibacterial functions of Ag nanoparticles. Antisense RNA, a single-stranded RNA, can hybridize with complementary genes in messenger RNA (mRNA) followed by blockage translation of these genes into proteins. Moreover, CRISPR (clustered regularly interspaced short palindromic repeats) is a family of viral DNA sequences derived from bacteriophages, which can target and destroy foreign DNA by nuclease activity. There are 2 classes and 6 subtypes (I-VI) of CRISPR-Cas systems, which may be engineered as potential antibacterial agents to target specific sequences. Therefore, here, recent advances and challenges for the antibacterial application of these three therapeutic agents are presented.
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Recently, stem cells have been considered renewable cell sources in the treatment of diabetes and the development of insulin-producing cells. In this regard, the current study aimed to compare Insulin-producing cells from bone marrow... more
Recently, stem cells have been considered renewable cell sources in the
treatment of diabetes and the development of insulin-producing cells. In
this regard, the current study aimed to compare Insulin-producing cells
from bone marrow stem cells with injectable insulin in rats with type I
diabetes. For this purpose, 40 rats were divided into four groups: the
control or healthy group, the diabetic control group, the group that
received differentiated insulin-producing cells from bone marrow, and
the group that received insulin treatment. To differentiate insulin-
producing cells from bone marrow, the femoral bone marrow of rats was
extracted using the flushing method. Differentiated cells were evaluated
using dithizone-specific dye, anti-insulin-proinsulin antibodies, and anti-
insulin beta receptors. Also, the expression of the pdx-I gene, as the
specific gene of pancreatic cells, was examined by RT-PCR. The results
showed that transplantation of insulin-producing cells could significantly
increase blood insulin levels in diabetic rats. This increase intensified in
the second stage of transplantation when more cells were injected into
rats. Concerning decreasing blood sugar levels, differentiated cells were
able to reduce blood sugar levels significantly. Even in the first stage of
cell injection, in which the rats received a small number of cells, their
blood sugar levels were controlled by these cells. As a result, the present
study showed that repeated transplants of insulin-producing cells
differentiated from bone marrow could decrease blood sugar and
increase insulin levels
treatment of diabetes and the development of insulin-producing cells. In
this regard, the current study aimed to compare Insulin-producing cells
from bone marrow stem cells with injectable insulin in rats with type I
diabetes. For this purpose, 40 rats were divided into four groups: the
control or healthy group, the diabetic control group, the group that
received differentiated insulin-producing cells from bone marrow, and
the group that received insulin treatment. To differentiate insulin-
producing cells from bone marrow, the femoral bone marrow of rats was
extracted using the flushing method. Differentiated cells were evaluated
using dithizone-specific dye, anti-insulin-proinsulin antibodies, and anti-
insulin beta receptors. Also, the expression of the pdx-I gene, as the
specific gene of pancreatic cells, was examined by RT-PCR. The results
showed that transplantation of insulin-producing cells could significantly
increase blood insulin levels in diabetic rats. This increase intensified in
the second stage of transplantation when more cells were injected into
rats. Concerning decreasing blood sugar levels, differentiated cells were
able to reduce blood sugar levels significantly. Even in the first stage of
cell injection, in which the rats received a small number of cells, their
blood sugar levels were controlled by these cells. As a result, the present
study showed that repeated transplants of insulin-producing cells
differentiated from bone marrow could decrease blood sugar and
increase insulin levels
Research Interests:
Rivaroxaban is an anticoagulant drug that prevents forming of blood clots. In addition, it can be administered to prevent and treat thrombotic diseases such as atrial fibrillation, cardiac arrhythmia, heart valve disease, orthopedic... more
Rivaroxaban is an anticoagulant drug that prevents forming of blood
clots. In addition, it can be administered to prevent and treat thrombotic
diseases such as atrial fibrillation, cardiac arrhythmia, heart valve
disease, orthopedic surgery, and thrombophilia to reduce the risk of
thrombosis. Various factors such as age, gender, diet, medications, and
genetic factors effectively determine the dose of rivaroxaban. Genetic
variability in drug-metabolizing enzymes, including the cytochrome
P450 (CYP450) enzymes and especially CYP3A4, has been associated
with rivaroxaban response. The current study aimed to identify the
frequency of CYP3A4 common polymorphisms, as well as their
association with rivaroxaban response in 100 patients of Arab descent
(48.6% female). CYP3A4 gene polymorphisms were examined by the
PCR-RFLP method, and the findings were analyzed by SPSS 16 software
and t-test. The frequency of CYP3A4*1B/*1B, CYP3A4*1B/*1A,
CYP3A4*1B/*1C, and CYP3A4*1A/*1C was 67.35%, 10.64%, 19.12% and
2.89%, respectively. According to our results, CYP3A4 *1B/*1B genotype
was the most common, and patients with CYP3A4*1B/*1B alleles needed
a higher daily dose of rivaroxaban than *1B/*1A, *1B/*1C, and *1A/*1C
carriers (9.57 ± 1.54 mg/day, P=0.015). Therefore, according to the
results, CYP3A4 gene polymorphism has an important effect on the dose
of rivaroxaban required to maintain the International Normalized Ratio
(INR) in the range of 2-3
clots. In addition, it can be administered to prevent and treat thrombotic
diseases such as atrial fibrillation, cardiac arrhythmia, heart valve
disease, orthopedic surgery, and thrombophilia to reduce the risk of
thrombosis. Various factors such as age, gender, diet, medications, and
genetic factors effectively determine the dose of rivaroxaban. Genetic
variability in drug-metabolizing enzymes, including the cytochrome
P450 (CYP450) enzymes and especially CYP3A4, has been associated
with rivaroxaban response. The current study aimed to identify the
frequency of CYP3A4 common polymorphisms, as well as their
association with rivaroxaban response in 100 patients of Arab descent
(48.6% female). CYP3A4 gene polymorphisms were examined by the
PCR-RFLP method, and the findings were analyzed by SPSS 16 software
and t-test. The frequency of CYP3A4*1B/*1B, CYP3A4*1B/*1A,
CYP3A4*1B/*1C, and CYP3A4*1A/*1C was 67.35%, 10.64%, 19.12% and
2.89%, respectively. According to our results, CYP3A4 *1B/*1B genotype
was the most common, and patients with CYP3A4*1B/*1B alleles needed
a higher daily dose of rivaroxaban than *1B/*1A, *1B/*1C, and *1A/*1C
carriers (9.57 ± 1.54 mg/day, P=0.015). Therefore, according to the
results, CYP3A4 gene polymorphism has an important effect on the dose
of rivaroxaban required to maintain the International Normalized Ratio
(INR) in the range of 2-3
Research Interests:
Naproxen is a common analgesic and antipyretic medication that is widely used around the world. This medicine at high doses leads to liver and kidney necrosis in humans and animals. The mechanism of kidney damage, unlike liver damage,... more
Naproxen is a common analgesic and antipyretic medication that is
widely used around the world. This medicine at high doses leads to liver
and kidney necrosis in humans and animals. The mechanism of kidney
damage, unlike liver damage, is not well understood and is one of the
most common causes of emergency department patients. Therefore, in
the present study, the protective effect of curcumin, a compound derived
from turmeric, was investigated on renal damage caused by naproxen.
For this purpose, 25 male Wistar rats were selected and were randomly
divided into five groups. Naproxen was dissolved in a 5% dimethyl
sulfoxide (DMSO) solution and was injected intraperitoneally at 1000
mg/kg of animal weight. Also, curcumin was dissolved in 5% DMSO and
was injected within peritoneum at a dose of 200 mg/kg of animal weight
into the relevant groups. After 24 hours of injection, rats were bled and
plasma urea and creatinine levels were measured. The rate of lipid
peroxidation, the activity of superoxide dismutase and catalase in the
kidney, total plasma antioxidant capacity, and PGC-1α gene expression
were measured. The results showed that naproxen significantly
increased the levels of biochemical markers of urea and creatinine in
plasma and lipid peroxidation in the kidney; also, it decreased the
activity of the antioxidants enzymes. The use of curcumin in naproxen-
exposed groups significantly reduced the concentrations of urea,
creatinine, and lipid peroxidation. Curcumin increased the activity of
catalase, superoxide enzymes, and the total antioxidant capacity of
plasma. Also, curcumin increased the expression of the PGC-1α gene,
which reduces the effects of naproxen. Therefore, according to the
current study results, curcumin could significantly reduce the harmful
effects of naproxen on the kidneys. However, in future studies, the effect
of curcumin should be evaluated on the naproxen mechanism in the
treatment of those patients who need naproxen.
widely used around the world. This medicine at high doses leads to liver
and kidney necrosis in humans and animals. The mechanism of kidney
damage, unlike liver damage, is not well understood and is one of the
most common causes of emergency department patients. Therefore, in
the present study, the protective effect of curcumin, a compound derived
from turmeric, was investigated on renal damage caused by naproxen.
For this purpose, 25 male Wistar rats were selected and were randomly
divided into five groups. Naproxen was dissolved in a 5% dimethyl
sulfoxide (DMSO) solution and was injected intraperitoneally at 1000
mg/kg of animal weight. Also, curcumin was dissolved in 5% DMSO and
was injected within peritoneum at a dose of 200 mg/kg of animal weight
into the relevant groups. After 24 hours of injection, rats were bled and
plasma urea and creatinine levels were measured. The rate of lipid
peroxidation, the activity of superoxide dismutase and catalase in the
kidney, total plasma antioxidant capacity, and PGC-1α gene expression
were measured. The results showed that naproxen significantly
increased the levels of biochemical markers of urea and creatinine in
plasma and lipid peroxidation in the kidney; also, it decreased the
activity of the antioxidants enzymes. The use of curcumin in naproxen-
exposed groups significantly reduced the concentrations of urea,
creatinine, and lipid peroxidation. Curcumin increased the activity of
catalase, superoxide enzymes, and the total antioxidant capacity of
plasma. Also, curcumin increased the expression of the PGC-1α gene,
which reduces the effects of naproxen. Therefore, according to the
current study results, curcumin could significantly reduce the harmful
effects of naproxen on the kidneys. However, in future studies, the effect
of curcumin should be evaluated on the naproxen mechanism in the
treatment of those patients who need naproxen.
Research Interests:
Glioblastoma is a fatal brain tumor, and the standard treatment for this cancer is the surgical removal of the tumor followed by chemotherapy with temozolomide and radiotherapy. Because chemotherapy has many side effects, the use of... more
Glioblastoma is a fatal brain tumor, and the standard treatment for this
cancer is the surgical removal of the tumor followed by chemotherapy
with temozolomide and radiotherapy. Because chemotherapy has many
side effects, the use of compounds extracted from natural herbs, due to
fewer side effects, can be a good alternative or supplement to chemical
drugs in cancer treatment. In this study, curcumin (diferuloylmethane),
known as the main active ingredient of turmeric, was used to evaluate its
cytotoxicity on four human glioblastoma cell lines (U373, U251, D54, and
T98G). Among these cell lines, U373 was temozolomide resistance, and
T98G was photodynamic treatment resistance. These cell lines were
treated with increasing concentrations of diferuloylmethane. Survival
percentage was assessed by MTT assay and the trypan blue staining
method was used to evaluate the rate of cell death and confirm the
results of the MTT assay. The results showed that diferuloylmethane has
a cytotoxic effect on U251, D54, and T98G cell lines. This effect was
higher in high concentrations of diferuloylmethane on U251 and D54
than on U373. Therefore, according to the results of the current study
and further studies, curcumin (diferuloylmethane) can be considered an
effective complementary treatment in the treatment of glioblastoma.
cancer is the surgical removal of the tumor followed by chemotherapy
with temozolomide and radiotherapy. Because chemotherapy has many
side effects, the use of compounds extracted from natural herbs, due to
fewer side effects, can be a good alternative or supplement to chemical
drugs in cancer treatment. In this study, curcumin (diferuloylmethane),
known as the main active ingredient of turmeric, was used to evaluate its
cytotoxicity on four human glioblastoma cell lines (U373, U251, D54, and
T98G). Among these cell lines, U373 was temozolomide resistance, and
T98G was photodynamic treatment resistance. These cell lines were
treated with increasing concentrations of diferuloylmethane. Survival
percentage was assessed by MTT assay and the trypan blue staining
method was used to evaluate the rate of cell death and confirm the
results of the MTT assay. The results showed that diferuloylmethane has
a cytotoxic effect on U251, D54, and T98G cell lines. This effect was
higher in high concentrations of diferuloylmethane on U251 and D54
than on U373. Therefore, according to the results of the current study
and further studies, curcumin (diferuloylmethane) can be considered an
effective complementary treatment in the treatment of glioblastoma.
Research Interests:
Breast cancer is one of the most common cancers known, and it is also a significant cause of death in women. If breast cancer is diagnosed in the early stages of the disease and treated appropriately, we can see an increase in life... more
Breast cancer is one of the most common cancers known, and it is
also a significant cause of death in women. If breast cancer is
diagnosed in the early stages of the disease and treated
appropriately, we can see an increase in life expectancy for more
than 90% of patients. Research on molecular biomarkers with
enough sensitivity and specificity can be a good solution for rapid
diagnosis in the clinical stage. Meanwhile, endogenous retroviral
biomarkers can have good functional benefits. Human Endogenous
Retroviruses as heterochromatin fragments of the genome usually
lack expression, but in several types of human cancers, including
breast cancer, HERV-Kenv mRNA is significantly increased. This
study used RT-PCR to detect the expression of HERV-K mRNA and
tried to introduce screening tools for the early detection of breast
cancer. In this case-control study, blood samples of 50 patients with
hospitalized breast cancer and 50 healthy individuals were designed
to evaluate the expression of HERV-Kenv mRNA using specific
primers and were analyzed by RT-PCR. PCR test was optimized as a
positive control using Hela cancer cell line (cervical
adenocarcinoma), which expresses the HERV-Kenv gene. Studies on
both patient and control groups showed that the increase in mRNA
expression was positive in 64% of patients with breast cancer and
negative in all healthy individuals. The results indicate an increase
in the expression of endogenous human retroviruses (HERVs) in
breast cancer. Because the amount of HERV-Kenv mRNA in the
blood of breast cancer patients increases dramatically, it is
predicted that these mobile genetic elements could be used as a
diagnostic biomarker.
also a significant cause of death in women. If breast cancer is
diagnosed in the early stages of the disease and treated
appropriately, we can see an increase in life expectancy for more
than 90% of patients. Research on molecular biomarkers with
enough sensitivity and specificity can be a good solution for rapid
diagnosis in the clinical stage. Meanwhile, endogenous retroviral
biomarkers can have good functional benefits. Human Endogenous
Retroviruses as heterochromatin fragments of the genome usually
lack expression, but in several types of human cancers, including
breast cancer, HERV-Kenv mRNA is significantly increased. This
study used RT-PCR to detect the expression of HERV-K mRNA and
tried to introduce screening tools for the early detection of breast
cancer. In this case-control study, blood samples of 50 patients with
hospitalized breast cancer and 50 healthy individuals were designed
to evaluate the expression of HERV-Kenv mRNA using specific
primers and were analyzed by RT-PCR. PCR test was optimized as a
positive control using Hela cancer cell line (cervical
adenocarcinoma), which expresses the HERV-Kenv gene. Studies on
both patient and control groups showed that the increase in mRNA
expression was positive in 64% of patients with breast cancer and
negative in all healthy individuals. The results indicate an increase
in the expression of endogenous human retroviruses (HERVs) in
breast cancer. Because the amount of HERV-Kenv mRNA in the
blood of breast cancer patients increases dramatically, it is
predicted that these mobile genetic elements could be used as a
diagnostic biomarker.
Research Interests:
Glucose-6-phosphate dehydrogenase (G6PD) is one of the most common genetic deficiencies that affect approximately 400 million people worldwide. This study aimed to identify neonates with G6PD deficiency in Ardabil province during... more
Glucose-6-phosphate dehydrogenase (G6PD) is one of the most
common genetic deficiencies that affect approximately 400 million
people worldwide. This study aimed to identify neonates with G6PD
deficiency in Ardabil province during 2017-2018. This cross-
sectional study was conducted on all term and preterm newborns in
Ardabil Province from April 2018 to April 2019. The sampling
method was census and in study duration, 1044 newborns were
entered in the study. For each infant, severe hyperbilirubinemia
(total serum bilirubin equal or greater than 300 micromol/L) was
tested by the diazo method and G6PD was evaluated by Fluorescent
Spot Test (FST). Of all infants, 15 (1.4 %) were diagnosed to have
G6PD deficiency by FST. The prevalence of G6PD deficiency was
significantly in boys higher than in girls (80% vs. 20%, p=0.001). Of
all infants, 97 (9.3%) had jaun dice 72 hours after birth that of them
7 neonates (7.2%) had G6PD deficiency. Results showed that the
prevalence of G6PD deficiency in this study was less than in other
places in Iran that may be because of different ethnicity and
demographic features.
common genetic deficiencies that affect approximately 400 million
people worldwide. This study aimed to identify neonates with G6PD
deficiency in Ardabil province during 2017-2018. This cross-
sectional study was conducted on all term and preterm newborns in
Ardabil Province from April 2018 to April 2019. The sampling
method was census and in study duration, 1044 newborns were
entered in the study. For each infant, severe hyperbilirubinemia
(total serum bilirubin equal or greater than 300 micromol/L) was
tested by the diazo method and G6PD was evaluated by Fluorescent
Spot Test (FST). Of all infants, 15 (1.4 %) were diagnosed to have
G6PD deficiency by FST. The prevalence of G6PD deficiency was
significantly in boys higher than in girls (80% vs. 20%, p=0.001). Of
all infants, 97 (9.3%) had jaun dice 72 hours after birth that of them
7 neonates (7.2%) had G6PD deficiency. Results showed that the
prevalence of G6PD deficiency in this study was less than in other
places in Iran that may be because of different ethnicity and
demographic features.